Intracellular delivery platform – Expanding the Boundaries of Biologics

Biond has developed INspire, an intracellular delivery platform for Biologics. This platform technology delivers various types of protein-based biologics, e.g. antibodies, nanobodies or enzymes, inside cells, allowing to drug intracellular-targets currently considered "undruggable". Using INspire, we have successfully delivered agents targeting KRAS, BRAF and HPV-E7, overcoming the fundamental challenges of the intracellular delivery of biologics. We have further showed intracellular functionality of all delivered agents

Challenges of intracellular delivery and the INspire solution

The INspire delivery technology is based on a chemically modified carrier protein which is further chemically conjugated to a biologic payload (antibodies, etc.). The carrier’s modification is characterized by a high and concentrated positive charge that enables cell internalization and efficient endosomal escape, delivering the payloads to the cytoplasm. The positive charges are further transiently masked, neutralizing the positive charges and adding negative charges, to enable clinically relevant PK and biodistribution.

Inspire Masking Technology

Delivering biologics to the tissue of interest and internalization of the biological payload into cells is a major challenge. INspire uses positive charges that are transiently masked by negative charges that are further removed in Low pH environments, such as can be found in the Tumor Microenvironment (TME) and inflammation sites. This design enables effective tissue targeting, intracellular delivery to target cells and endosomal escape

Athymic nude mice inoculated with HeLa-GFP cells. Red – Carrier, Blue – Nuclei of all cells